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Optimization of Medium Components for the Production of Raw-Starch-Digesting Amylase by Monascus M2 in Solid State Fermentation

LIU Bo, ZENG Li-ping, WU Ying-long   

  1. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
  • Online:2014-04-15 Published:2014-04-18

Abstract:

The activity of raw-starch-digesting amylase produced by Monascus M2 in solid state fermentation wasinvestigated with respect to three medium components including carbon source, nitrogen source and inorganic salt. Responsesurface methodology was used to optimize three different levels of lactose, (NH4)2SO4 and K2HPO4 by setting up a quadraticregression model. The optimized basic solid medium contained 8.18% lactose, 6.36% (NH4)2SO4 and 0.91% K2HPO4. Theresulting maximum predicted activity of raw-starch-digesting amylase was 680.29 U/g, compared to 662.21 U/g observed invalidation experiments.

Key words: Monascus, solid-state fermentation, raw-starch-digesting amylase, Box-Behnken experiment design