Establishment and Application of an Analytical Database for Cronobacter spp. by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

doi:10.7506/spkx1002-6630-201408020

Abstract

Abstract:

The purpose of this study was to establish an analytical database for high throughput identification and subtyping
of 8 species of Cronobacter spp. by a matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDITOF-
MS) method. Under the optimized culture and sample treatment conditions by using the established MALDI-TOFMS
protein fingerprint acquisition parameters, the acquired characteristic protein mass spectrometric profiles of 8 reference
strains were used to build a MALDI-TOF-MS database with Biotyper software, and the accuracy of the database was
validated by comparison with data from related enterobacteriaceae strains and isolates of the genus Cronobacter. The
MALDI-TOF-MS database was applied to analyze 135 isolates of Cronobacter spp. with an identification score no smaller
than 2.0, which reached the requirement for identification at the species level. Further subtyping was achieved by cluster
analysis. This MALDI-TOF-MS database may provide a new approach for high throughput identification and subtyping of
Cronobacter strains.

Key words: Cronobacter spp., MALDI-TOF-MS, database, identification, subtyping

CLC Number:

TS207.4

ZHAO Gui-ming, LIU Yang, CHEN Ying*, YANG Hai-rong, ZHAO Yong-sheng, WANG Ping. Establishment and Application of an Analytical Database for Cronobacter spp. by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201408020.

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Establishment and Application of an Analytical Database for Cronobacter spp. by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

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