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Chemical Composition of Xiaguan Raw Tuocha and Its Preventive Activity on HCl/Ethanol-Induced Gastric Injury in SD Rats

WANG Rui, ZHAO Xin   

  1. Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
  • Online:2014-07-15 Published:2014-07-18

Abstract:

Xiaguan Tuocha is a functional drink and known to possess some health properties. In order to identify the
chemical components and investigate preventive effects of Xiaguan raw (unfermented) Tuocha (XRT) on gastric injury
in vivo, liquid chromatography-mass spectrometry (LC-MS) and cytokine analysis were used in this study, and the gastric
secretion and pH of gastric juice were also tested. Twelve components were found in XRT, all of which contributed to the
preventive effect on gastric injury. XRT at higher concentration reduced the levels of serum proinflammatory cytokines such
as IL-6 and TNF-α compared with at lower concentration. The 1 000 mg/kg XRT showed the best inhibitory effect on gastric
injury (78.7% inhibitory rate). The 1 000 mg/kg XRT treated rats also exhibited similarly low gastric secretion (2.0 mL) and
higher pH of gastric juice (pH 3.1) compared with normal rats. Xiaguan Tuocha contained many functional components and
exerted a strong preventive effect on gastric injury.

Key words: Tuocha, gastric injury, cytokine, rat