Abstract:

The fruit bodies of Termitomyces were collected, and total DNA was extracted as the template to amplify internal
transcribed spacer (ITS). Subsequently, the PCR products were sequenced, and the phylogenetic tree of Termitomyces was
established based on different parts of ITS region by the MEGA5 software. The results showed that the rDNA-ITS region
of 10 different species of Termitomyces was between 527 bp and 661 bp in length. The phylogenetic tree showed that there
were eight branches of Termitomyces. T. clypeatus and T. globules were gathered together. The unknown species A1, E1 and
H1 which were in a single branch may be new species. B1, G1, and D1 could be identified as T. heimii. In addition, I1 could
be T. eurrhizus, and ZZ1 could be T. robustus. However, C1 could not be determined based on the present study. The results
showed that the region of ITS1 and ITS1-5.8S rDNA-ITS2 could be used to build the phylogenetic tree for interspecific
Termitomyces. The phylogenetic tree of ITS1 had a significantly higher supporting rate, and the ITS2 could be helpful
for the confirmation of some unknown species. Therefore, this work has laid a foundation for the classification system of
Termitomyces and provided ITS region sequence information for the identification at the molecular level.

Key words: Termitomyces, phylogenetic tree, ITS region