Analysis of Genetic Diversity of Ten Cultivated Strains of Pleurotus nebrodensis

doi:10.7506/spkx1002-6630-200905036

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (5): 158-161.doi: 10.7506/spkx1002-6630-200905036

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Analysis of Genetic Diversity of Ten Cultivated Strains of Pleurotus nebrodensis

SU Hong-yan，WANG Lei*，WANG Zhong-li，WANG Dan

(College of Life Sciences, Ludong University, Yantai 264025, China)

Received:2008-03-26 Revised:2008-07-05 Online:2009-03-01 Published:2010-12-29
Contact: WANG Lei*， E-mail:wanglei9909@163.com

Abstract

Abstract:

Diversity of genomic DNA of ten cultivated strains of Pleurotus nebrodensis was analyzed by RAPD (random amplified polymorphic DNA). 52 DNA fragments were amplified from 4 selected primers, among which 44 were polymorphic bands with 84.6% of polymorphic site frequency. The result indicated that the DNA fingerprint profiles of the ten strains of Pleurotus nebrotensis are greatly diverse. Moreover, the genetic distance among strains was calculated and the phylogenetic tree was constructed by Popgen32 software. Further, the genetic relationship of the ten tested strains was identified on molecular level, which can provide important scientific basis for accurate classification of Pleurotus nebrotensis.

Key words: RAPD, Pleurotus nebrodensis, genetic diversity, phylogenetic tree

CLC Number:

S646

SU Hong-yan，WANG Lei*，WANG Zhong-li，WANG Dan. Analysis of Genetic Diversity of Ten Cultivated Strains of Pleurotus nebrodensis[J]. FOOD SCIENCE, 2009, 30(5): 158-161.

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Analysis of Genetic Diversity of Ten Cultivated Strains of Pleurotus nebrodensis

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