Abstract:

Crude glycoproteins (PDGs) were obtained from Polygonatum odoratum through phosphate buffer extraction.
PDGs were further purified by Sephadex G-100 gel filtration and concanavalin A affinity chromatography, and PDG1
and PDG3 were acquired. The molecular weights and antioxidant activity of these two fractions were determined. The
results showed that PDG1 and PDG3 had a molecular weight of 186 and 28.3 kD, respectively, and PDG, PDG1 and PDG3
had reducing power, strong abilities to scavenge hydroxyl radical and 1,1-diphenyl-2-picrylhydrazy (DPPH) radical and
inhibitory effects against lipid peroxidation, showing a dose-effect relationship in the range of 1–10 mg/mL. Generally,
PDG1 and PDG3 exhibited better antioxidant activity than PDGs.

Key words: Polygonatum odoratum, Sephadex G-100, concanavalin A, glycoprotein, antioxidant activity