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Optimization of Extraction Process for Huperzine A from Huperzia serrata

YANG Ting1,2,4, WU Yun1,2,*, ZHANG Ze3, WU Yulian3, ZHENG Xiaojiang1,2, HU Shikun2, MAO Xin2   

  1. 1. Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province, Hubei Institute for Nationalities,
    Enshi 445000, China; 2. College of Biological Scientific and Technical, Hubei University for Nationalities,
    Enshi 445000, China; 3. University Hospital of Hubei University for Nationalities, Enshi 445000, China;
    4. College of Life Sciences, Sichuan University, Chengdu 610064, China
  • Online:2015-01-25 Published:2015-01-16
  • Contact: WU Yun

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Abstract:

This paper is concerned with the selection and optimization of important extraction parameters as well as their
levels for the increased yield of huperzine A from the aboveground parts of Huperzia serrata by combined use of single
factor method and response surface methodology (RSM). Solid-to-solvent ratio, solvent concentration and temperature were
identified as main variables that affect extraction efficiency, and their levels were optimized using RSM based on a threevariable,
three-level Box-Behnken design. When the extraction was performed for 4 h under the optimized conditions: 1.5%
tartaric acid as extraction solvent with a solid-to-solvent ratio of 1:30 (g/mL) and an extraction temperature of 41 ℃, the
yield of huperzine A was 11.192 8 mg/100 g.

Key words: Huperzia serrata, huperzine A, response surface analysis, high performance liquid chromatography

CLC Number: