Abstract:

The enzymatic hydrolysis of cotton seed husk with xylanase for xylo-oligosaccharides production was optimized
by response surface methodology in this research. A pretreatment time of 35.70 min with 0.17% HCl was found optimal
for the enzymatic hydrolysis, which was performed at an initial pH of 6.5. At the end of the hydrolysis, the concentration
of xylobiose in hydrolysis was measured to be 14.52 g/L, agreeing with the predicted value. The final product was mostly
xylobiose with a yield of 8.13% (relative to the dry weight of cotton seed husk).

Key words: cottonseed husk, xylo-oligosaccharides, xylobiose, response surface methodology