Overexpression of the Lipase Gene from Kurtzmanomyces in Pichia pastoris

doi:10.7506/spkx1002-6630-201503020

Abstract

Abstract:

A stable and highly effective expression system was constructed for the expression of the lipase gene from
Kurtzmanomyces sp. V3. The lipase gene was isolated by homologous cloning, inserted into the expression vector pPICZαA
and transformed into Pichia pastoris X33. The results showed that the gene was expressed successfully in Pichia pastoris X33.
The optimal temperature and pH of the recombinant lipase were 60 ℃ and 5.0, respectively. Its activity was stimulated by
1 mmol/L Mg2+, K+ and Na+, and 1 g/100 mL Triton-100, Tween-80 and Tween-20. The recombinant lipase showed high
activity toward tristearin (C18).

Key words: Kurtzmanomyces, lipase, Pichia pastoris

CLC Number:

WANG Jianrong, LIU Danni, LI Peng, LI Yangyuan. Overexpression of the Lipase Gene from Kurtzmanomyces in Pichia pastoris[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201503020.

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Overexpression of the Lipase Gene from Kurtzmanomyces in Pichia pastoris

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