Abstract:

Ultrasound-assisted extraction was adopted to extract water-soluble proteins (WSP) from Corbicula fluminea.
The optimization of four extraction parameters including solid to liquid ratio, extraction time, extraction temperature,
and ultrasound power for the enhanced yield of water-soluble proteins was carried out using Box-Behnken design (BBD)
and response surface methodology (RSM). Moreover, the antioxidant potential of WSPs from Corbicula fluminea was
investigated. The optimal extraction conditions that provided maximum WSP yield (67.70%) were determined as follows:
solid to liquid ratio, 1:20; extraction time, 23 min; extraction temperature, 48 ℃; and ultrasonic power, 920 W. The analysis
of variance (ANOVA) showed that the effects of extraction time and ultrasound power on WSP yield were significant. The
cells were broken more significantly by ultrasonic cavitation and vibration to fully expose the target product to the solvent,
accordingly increasing the extraction efficiency and shortening the extraction time. Thus, the application of ultrasonic is of
great significance for water-soluble protein extraction. The scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH)
and superoxide anion radicals and ferric ion reducing power of the extracts were strong, but the hydroxyl radical scavenging
activity was weak.

Key words: Corbicula fluminea, proteins, ultrasound, extraction, antioxidant activity