Preparation and Characterization of Fluorene Complete Antigen and Anti-fluorene Polyclonal Antibody

doi:10.7506/spkx1002-6630-201509020

Abstract

Abstract:

Fluorene-9-acetic acid (FLU) was chosen as the hapten and was coupled with bovine serum albumin (BSA)
and ovalbumin (OVA) respectively to prepare complete antigens by active ester method. The complete antigens were
characterized by native polyacrylamide gel electrophoresis and ultraviolet spectrum. FLU-BSA was used to obtain
polyclonal antibody through footpad injection of BALB/c mice. The titer was 51 200 as measured by indirect enzyme linked
immunosorbent assay (ELISA), and the cross-reactivity with fluoranthene was measured by indirect competitive ELISA
to be 12% while having no obvious cross-reaction with 14 other kinds of polycyclic aromatic hydrocarbons (PAHs). This
ELISA method for fluorene can lay the foundation for further experimental research.

Key words: fluorene-9-acetic acid, complete antigen, polyclonal antibody, enzyme linked immunosorbent assay (ELISA)

CLC Number:

TS201.6

WANG Nannan, LI Yansong, MENG Xingyu, ZHOU Yu*, QIAO Bin, SUN Yu, HU Pan, LU Shiying, REN Honglin, LIU Zengshan. Preparation and Characterization of Fluorene Complete Antigen and Anti-fluorene Polyclonal Antibody[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201509020.

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Preparation and Characterization of Fluorene Complete Antigen and Anti-fluorene Polyclonal Antibody

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