Abstract: An indirect competitive chemiluminescene enzyme immunoassay (icCLEIA) for the rapid determination of isotenuazonic acid (ITeA) in fruit juice and tomato sauce was developed. The optimized experimental parameters were obtained as follows: the coating antigen concentration was 15.6 ng/mL, the antibody was diluted 96 000 folds, the buffer pH was 7.4, and the time of competition and anti-mouse IgG reaction were 40 and 50 min, respectively. The half maximal inhibitory concentration (IC50) of the proposed method was 2.13 ng/mL with limit of detection (LOD) of 0.10 ng/mL in the linear range from 0.50 to 9.12 ng/mL. The cross-reactivity with ITeA analogues was lower than 0.1%. The recoveries of ITeA from spiked samples ranged from 78.60% to 110.83%. The coefficient of variation was lower than 15%. This proposed icCLEIA provided an efficient method for rapid detection of ITeA in real samples.

Key words: mycotoxin, iso-tenuazonic acid (ITeA), polyclonal antibody, chemiluminescene enzyme immunoassay, rapid detection