Abstract:

One Lactobacillus strain isolated from homemade cherry wine revealed significant inhibitory activity
against Gram-positive and Gram-negative bacteria using double plate punching method, which was named LD 1.0008.
LD 1.0008 was identified as Lactobacillus plantarum by its carbohydrate fermentation pattern using API 50 CHL test kits,
16S rDNA sequence analysis and recA-based polymerase chain reaction (PCR) test. The antibacterial activity was
significantly decreased after protease treatment, whereas it remained stable after treatment with catalase. The antibacterial
substances were confirmed as bacteriocin after excluding the interference of organic acid. PCR amplification was carried out
using previously reported 10-pair plantaricin primers. It was indicated that at least 4 bacteriocin-encoding genes could be
produced by LD 1.0008, including plnD, plnO, plnV and plnW. It is of great significance for the separation and application of
plantaricins of LD 1.0008.

Key words: bacteriocin, Lactobacillus, identification, bacteriocin-encoding genes