Methodology Validation for Determination of L-Zinc Lactate by High Performance Liquid Chromatography with A Chiral Column

doi:10.7506/spkx1002-6630-201514021

Abstract

Abstract:

This paper describes the development of a validated method for determining L-zinc lactate by high performance
liquid chromatography (HPLC). An ideal chromatographic separation between D-zinc lactate and L-zinc lactate was achieved
on an OA-5000 chiral column using 1 mmol/L CuSO4·5H2O as mobile phase at a flow rate of 1 mL/min. The established
calibration curve showed a good linearity over the concentration range of 0.147 to 3.675 mg/mL, with a correlation
coefficient of 0.999 9 (n = 6). The relative standard deviation (RSD) for precision and stability were respectively 0.044%
was and 0.061%, and the average recovery was (99.8 ± 1.90)% with RSD of 1.90%. It is shown that this method is simple,
accurate and reliable, and can be used to monitor the content of L-zinc lactate.

Key words: high performance liquid chromatography (HPLC), L-zinc lactate, chirality

CLC Number:

TS202.3

HE Yueming1, WANG Shan2, JIE Qinfeng2, QIU Weihua2, HUANG Xiaowan1, DENG Zeyuan1,*. Methodology Validation for Determination of L-Zinc Lactate by High Performance Liquid Chromatography with A Chiral Column[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201514021.

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Methodology Validation for Determination of L-Zinc Lactate by High Performance Liquid Chromatography with A Chiral Column

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