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Establishment of the Alphalisa Method for the Detection of Penicillinase in Milk Products

ZHANG Jianglin1,2, NIE Fuping1, YANG Jun1, LI Yingguo1, XIAO Jinwen1, CAI Jiali2, YUAN Zengzhuang3, YE Zixia3, WANG Guomin1, WANG Yu1,*   

  1. 1. Technical Center, Chongqing Entry-Exit Inspection and Quarantine Bureau, Chongqing 400020, China;
    2. Institute of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, China;
    3. College of Animal Science and Technology, Southwest University, Chongqing 400716, China
  • Online:2015-07-25 Published:2015-07-15
  • Contact: WANG Yu

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Abstract:

Penicillinase is one of the rick drugs present in milk products. In this article, the standard penicillinase was
biotinylated, and competitively bound to mouse anti-penicillinase monoclonal antibodies by reverse competitive Alphalisa.
The established Alphalisa detection method was shown to specifically identify penicillinase present in the samples. The
variations between batches were less than 10%, with the limit of detection and the limit of quantitation of 1.6 IU/mL and
5 IU/mL, respectively, and the linearity range of 2.5-500 IU/mL. The results show that this method can replace the
traditional ELISA method as a laboratory screening or detection method for penicillinase.

Key words: penicillinase, alphalisa detection, milk products

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