Abstract:

An ion chromatography coupled with conductivity detection method was developed for the determination of
betaine in Lycium ruthenicum Murr.. The analyte was separated on a Metrosep C4 cation exchange column (150 mm ×
4.6 mm, 4 μm) by using methane sulfonic acid (1.5 mmol/L) as mobile phase. The flow rate was 0.8 mL/min at a column
temperature of 38.5 ℃. It was found that the linearity range of betaine was 10.4–830.0 mg/L (R2 = 0.999 8) and the spiked
recoveries varied in the range of 95.51%–98.06%. The proposed method was found to be simple, reproducible and suitable
for the determination of betaine in L. ruthenicum.

Key words: ion chromatography, Lycium ruthenicum Murr., betaine