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Optimization of Microwave-Assisted Extraction by Response Surface Analysis and Purification of Flavonoids from Choerospondias axillaris Fruit

YANG Yunshu, LI Rong, JIANG Zitao   

  1. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science,
    Tianjin University of Commerce, Tianjin 300134, China
  • Online:2015-11-25 Published:2015-12-03
  • Contact: JIANG Zitao

Abstract:

The optimal conditions for microwave-assisted extraction of flavonoids from the flesh of Choerospondias axillaris
fruits were established by using single factor experiments and response surface analysis as follows: extraction temperature,
75 ℃; ratio of solvent to material, 62:1 (mL/g); ethanol concentration, 62%; microwave power 500 W; and extraction time,
7 min. Under the proposed conditions, the yield of flavonoids was much higher than that obtained from some methods
reported in the literature. The optimal adsorption/desorption conditions for purifying the crude flavonoids with AB-8
macroporous adsorbent resin were determined as follows: the extract solution at pH 2 was passed through the column at a
flow rate of 2 bed volumes/h (BV/h), and then the column was eluted with 60% alcohol at 3 BV/h. The purification of crude
flavonoids from C. axillaris was also investigated using preparative chromatography and the feasibility was confirmed by
the results of HPLC analysis.

Key words: Choerospondias axillaris, response surface analysis, macroporous resin, preparative chromatography

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