Abstract:

Dekkera bruxellensis is one of the major spoilage microorganisms in wines. In order to detect Dekkera
bruxellensis in wines rapidly and accurately, the separation and detection efficiency of Dekkera bruxellensis using differential
medium (DBDM) and Wallerstein nutrient (WLN) medium were compared. A specific polymerase chain reaction (PCR)
assay was also used to further testify the results. The results showed that both DBDM and WLN could be useful to detect
Dekkera bruxellensis in wines, while DBDM was more selective to identify Dekkera bruxellensis accurately when combined
with the specific PCR. The application of DBDM combined with the specific PCR to investigate the infection of Dekkera
bruxellensis in wines from five major producing regions in China showed that among the investigated regions, Dekkera
bruxellensis spoilage was found in wines from three regions including Hebei, Shandong and Jilin, especially in the wines
aged in oak barrels. Therefore, the Chinese wine industry should pay much attention to the issue of Dekkera bruxellensis
spoilage.

Key words: wine, Dekkera bruxellensis, differential medium, detection and identification