Optimization of Fermentation Condition for an AFB1-Degrading Strain and Preliminary Exploration of Degradation Mechanism

doi:10.7506/spkx1002-6630-201605025

Abstract

Abstract:

In this study, a mould isolated for degrading aflatoxin B1 was reported. Morphological studies coupled with
internal transcribed spacer (ITS), 18S ribosomal RNA gene sequence, 26S ribosomal RNA gene sequence analyses
indicated that the mould was Cladosporium uredinicola. Through single-factor experiments, the fermentation conditions
were determined as follows: temperature 28 ℃, medium loading volume 75 mL/250 mL, inoculumamount 15% (V/V),
fermentation time 36 h, initial pH 8.0. Under these conditions, the AFB1 degradation efficiency was 68.96%, a 69.52%
increase over that before optimization (40.68%). On the thin-layer chromatography (TLC) plate, a new dot was found which
exhibited blue fluorescence at 365 nm.

Key words: aflatoxin B1 (AFB1), identification, fermentation condition, degradation product

CLC Number:

TS201.3

SHAO Shuai, DAI Jun, DU Xin, WANG Changgao, LIN Jianguo, CAI Jun. Optimization of Fermentation Condition for an AFB1-Degrading Strain and Preliminary Exploration of Degradation Mechanism[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201605025.

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Optimization of Fermentation Condition for an AFB1-Degrading Strain and Preliminary Exploration of Degradation Mechanism

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