Abstract:

In order to synthesize a novel and effective artificial paclobutrazol antigen, succinic anhydride method was used
to modify the structure of paclobutrazol. Paclobutrazol hapten (PAC-HS) with an active carboxyl group was obtained, and
conjugated separately with BSA (bovine serum albumin) and OVA (ovalbumin) by carbodiimide method. Immunogen
and coating antigen were obtained and designated as PAC-HS-BSA and PAC-HS-OVA, respectively. The hapten was
characterized by mass spectrometry, infrared spectroscopy and 1H nuclear magnetic resonance spectroscopy. Ultraviolet
spectrophotometry was applied to determine the artificial paclobutrazol antigen. The coupling ratios of the conjugates were
determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). The results
suggested that paclobutrazol antigen was successfully synthesized, which will greatly facilitate antibody production and
immunoassay development.

Key words: paclobutrazol, hapten, artificial antigen, preparation