FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (10): 292-297.doi: 10.7506/spkx1002-6630-20180323-304

• Safety Detection • Previous Articles     Next Articles

Evaluation of Uncertainty in Determination of Plant Growth Regulator Residues in Bean Sprouts by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

LI Jing, ZHANG Juzhou*, YU Xiaojuan, LIU Yi   

  1. Anhui Provincial Institute for Food and Drug Test, Hefei 230051, China
  • Online:2019-05-25 Published:2019-05-31

Abstract: The study was aimed at evaluation of the measurement uncertainty in the determination of three plant growth regulator residues in bean sprouts by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS). According to Evaluation and Expression of Uncertainty of Measurement (JJF 1059.1-2012), a mathematical model of uncertainty evaluation was constructed, and the sources of uncertainty were analyzed in depth, and each component was quantified and synthesized. The results showed that the main sources of uncertainty arose from the preparation of standard solution and curve fitting. Furthermore, the expanded uncertainty was 0.6, 2.3 and 2.3 μg/kg for paclobutrazol, indole-3-acetic acid and 3-indolebutyric acid contents of 5.9, 15.5 and 16.0 μg/kg in bean sprouts (k = 2), respectively.

Key words: ultra performance liquid chromatography-tandem mass spectrometry, paclobutrazol, indole-3-acetic acid, 3-indolebutyric acid, uncertainty, bean sprout

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