Microstructure and Immunomodulating Activity of Mucilage from “Tiegun” Yam Tubers (Dioscorea opposita Thunb. cv. Tsukune) and Its Hydrolysates

doi:10.7506/spkx1002-6630-201611011

Abstract

Abstract:

Mucilage was extracted from “Tiegun” yam tubers (Dioscorea opposita Thunb. cv. Tsukune), and its hydrolysates
were obtained by hydrolysis with different enzymes (trypsin, cellulase and complex protease). The microstructure of
mucilage and its hydrolysates were detected by infrared spectroscopy and scanning electron microscopy. Meanwhile, their
effects on the proliferation of spleen lymphocytes and the relative expression levels of cytokine mRNA were explored by cell
culture in vitro and quantitative real-time polymerase chain reaction (RT-PCR) methods, respectively. The results showed that
mucilage and its hydrolysates were different in microstructure. Mucilage and its hydrolysates could promote the proliferation
and transformation of spleen lymphocytes, but the promoting effect of mucilage was weaker than that of its hydrolysates.
Promoting effects of mucilage and its hydrolysates on the mRNA expression of Th1 cytokines were significantly higher than
that of Th2 cytokines. These results showed that yam mucilage and its hydrolysates had potential function to shift the Th1/Th2
balance to relative Th1 dominance.

Key words: yam mucilage, hydrolysates, lymphocyte transformation, cytokine, mRNA expression

CLC Number:

TS201.4

REN Guoyan, WU Tingting, ZHANG Fan, GUO Jinying, CUI Guoting, WU Ying, WANG Ping, CAO Li. Microstructure and Immunomodulating Activity of Mucilage from “Tiegun” Yam Tubers (Dioscorea opposita Thunb. cv. Tsukune) and Its Hydrolysates[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201611011.

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Microstructure and Immunomodulating Activity of Mucilage from “Tiegun” Yam Tubers (Dioscorea opposita Thunb. cv. Tsukune) and Its Hydrolysates

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