Abstract:

Based on the data collected with a high performance liquid chromatograph (HPLC)-spectrometer, vector-subspaceangle criterion was calculated. A new method was developed to rapidly analyze malachite green residues in aquatic products.Multi-wavelength UV-visible optical fiber spectrometer was applied firstly to set up a malachite green standard spectraldatabase and a spectral database for marketed aquatic product samples. Then using HPLC-spectrometer, a backgroundspectral database of malachite green in aquatic products was created. The vector-subspace angle criterion was appliedto calculate the contents of malachite green residues in aquatic products including grass carp, tilapia, yellow catfish, seacrab, shrimp and corn snail. The results showed that within a concentration range of 0.2–4.0 μg/mL, the linear correlationcoefficient for the standard curve was 0.999 7 (at 618 nm), and the recoveries of malachite green in spiked samples were99.24%–109.38%, with a relative standard deviation of less than 5.0%. Thus, this method can be used to rapidly andaccurately detect malachite green residues in aquatic products.

Key words: vector-subspace angle criterion, multi-wavelength UV-visible spectroscopy, high performance liquid chromatography (HPLC), malachite green