Optimization of Soybean Oil Degumming by Mixed Recombinant Phospholipases

doi:10.7506/spkx1002-6630-201618003

Abstract

Abstract:

Crude soybean oil was degummed by mixtures of recombinant phospholipases A1 (PLA1) and phospholipase
C (PLC), which were expressed successfully in the recombinant E. coli. by our laboratory. We investigated the effects of
different parameters, including temperature, pH, reaction time, water dosage, stirring rate and enzyme dosage on degumming
efficiency. Commercial PLA1 (Lecitase Ultra™) was used for comparison. Then, these parameters were optimized by
orthogonal array design. The optimal conditions were confirmed as follows: reaction temperature, 45 ℃; pH, 6.5; reaction
time, 3 h; stirring speed, 300 r/min; PLA1 dosage, 7 940 U/kg; and PLC dosage, 23 130 U/kg. The degumming efficiency
obtained with the mixed recombinant enzymes was comparable to that obtained with Lecitase Ultra™. The final phosphorus
content of degummed soybean oil was lower than 5 mg/kg, which met the requirements of physical refining. This study laid
the foundation for developing food-grade enzyme preparations with self-owned intellectual property rights of cooking oil
degumming.

Key words: recombinant E. coli, mixed phospholipases, soybean oil, enzymatic degumming, orthogonal array experiment

CLC Number:

Q814.9

CHENG Shi, WANG Changkun, ZHANG Liang, LI Ying, SHI Guiyang, YANG Shengrong, CHEN Guo’an. Optimization of Soybean Oil Degumming by Mixed Recombinant Phospholipases[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201618003.

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Optimization of Soybean Oil Degumming by Mixed Recombinant Phospholipases

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