Isolation and Identification of a Bacillus from Peach Tree Gum and Its Main Antimicrobial Substance

doi:10.7506/spkx1002-6630-201621024

Abstract

Abstract:

A strain of Bacillus named as TJ12 was isolated from peach tree gum, which could produce antibacterial
substances. Its fermentation products had antibacterial activity against Staphylococcus aureus, Micrococcus luteus, Bacillus
cereus and Fusarium moniliforme. Through colony morphology, physiological and biochemical characterization and 16S
rRNA and gyrB sequence analysis, the strain was identified as Bacillus licheniformis. The major bioactive substance was
separated from its fermentation supernatant and purified by ammonium sulfate precipitation, organic solvent extraction,
Sephadex LH-20 column chromatography and high performance liquid chromatography. The major bioactive substance had
strong resistance to pH, temperature, and three proteases (trypsin, proteinase K, and pepsin). By electrospray ionization-mass
spectrometry analysis, we preliminarily identified this substance as bacitracin A.

Key words: peach tree gum, Bacillus licheniformis, antimicrobial substances, separation and purification, identification, bacitracin A

CLC Number:

TS201.3

JIA Rui, LU Zhaoxin. Isolation and Identification of a Bacillus from Peach Tree Gum and Its Main Antimicrobial Substance[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201621024.

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Isolation and Identification of a Bacillus from Peach Tree Gum and Its Main Antimicrobial Substance

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