Identification of Anguilla rostrata, Anguilla anguilla and Anguilla japonica Using DNA
Barcoding Based on 16S rRNA Gene

doi:10.7506/spkx1002-6630-201704046

Abstract

Abstract: To meet the demands of enterprises engaged in eel farming, processing and trading and law enforcements, a DNA barcoding method for the identification of three main farmed eel species, Anguilla rostrata, A. Anguilla and A. japonica, was developed. Universal 16S rRNA primers were designed and used to amplify partial 16S rRNA gene fragments of A. rostrata, A. Anguilla and A. japonica and their PCR products were sequenced to be 638, 638 and 640 bp in length, respectively. The specific DNA fragment (243 bp) was selected as standard DNA barcode with eel species specificity to identify eel species conveniently, and a forward primer (22 bp) and a reversed primer (23 bp) were designed and used to establish the PCRDNA barcoding method. The results of the application of the proposed method over the past three years showed that it is convenient, accurate, stable, and useful to identify the three eel species.

Key words: A. rostrata, A. Anguilla, A. japonica, 16S rRNA gene, DNA barcode, identification

CLC Number:

S917.4
Q78

CHEN Wenbing, MIAO Tingyu, PENG Juan, SHAO Biying, CHEN Bin, JIANG Shuxun, ZHANG Zhideng. Identification of Anguilla rostrata, Anguilla anguilla and Anguilla japonica Using DNA Barcoding Based on 16S rRNA Gene[J]. FOOD SCIENCE, 2017, 38(4): 283-289.

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Identification of Anguilla rostrata, Anguilla anguilla and Anguilla japonica Using DNA Barcoding Based on 16S rRNA Gene

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