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The Alcoholic Extract of Capparis zeylanica Linn Protects against Oxidative Damage in Saccharomyces cerevisiae: An Investigation into the Mechanism

ZHANG Peng1, WANG Xiangxing2, YANG Yaping2, LI Shiming3, ZHAO Hui2, LUO Yanping1,*   

  1. 1. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China;
    2. Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin 300134, China;
    3. Hubei Provincial Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University, Huanggang 438000, China
  • Online:2017-04-15 Published:2017-04-24

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Abstract: The crude alcoholic extract of Capparis zeylanica Linn (AECZ) was purified through AB-8 macroporous resin. The in vitro tests indicated that AECZ had potent hydroxyl radical and (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activities. The cytoprotection of AECZ in Saccharomyces cerevisiae BY4741 and its homogenous strains deficient in antioxidant enzyme genes under oxidative stress induced by H2O2, CCl4 and CdSO4 was explored. Interestingly, AECZ could dramatically protect yeasts against oxidative stresses induced by H2O2, CCl4 and CdSO4. Adaptive treatment with AECZ could reduce the level of intracellular lipid peroxidation and reactive oxygen species in yeasts under stress conditions. Moreover, the GTT1 gene played an important role in alleviating oxidative damage in yeast cells. This study for the first time demonstrated the intracellular antioxidant activity of AECZ, which will provide theoretical evidence for further exploitation and utilization of Capparis zeylanica Linn.

Key words: Capparis zeylanica Linn, antioxidant, Saccharomyces cerevisiae, cellular oxidation, lipid peroxidation

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