Degradation of Aflatoxin B1 by a Microbial Consortium and Its Composition and Diversity

doi:10.7506/spkx1002-6630-201709017

Abstract

Abstract: External elimination method was applied to obtain an efficient aflatoxin B1-degrading microbial consortium, designated FBAD-2. The degradation rates of 2 000 and 5 000 μg/L AFB1 by the microbial consortium could reach 100% and 90% within 120 h, respectively. The toxin degradation ability of FBAD-2 was kept at a high level in the range of 30–70 ℃, with an optimal temperature of 60 ℃. The results of AFB1 detoxification experiment showed that the extracellular enzymes in the supernatant played a major role in the degradation of AFB1. Furthermore, the optimal culture time for enzyme production was 24 h, and the supernatant could completely degrade 5 000 μg/L AFB1 within 48 h. 16S rRNA gene clone library analysis showed that FBAD-2 was mainly composed of bacteria from the genera Geobacillus, Symbiobacterium thermopilum, Clostridium and Tepidanaerobacter.

Key words: aflatoxins B1, degradation, bacterial community, microbial composition and diversity

CLC Number:

X56
Q939.5

ZHAO Chunxia, WANG Yi, CHENG Wei, Lü Yucai, GONG Dachun, GUO Peng. Degradation of Aflatoxin B1 by a Microbial Consortium and Its Composition and Diversity[J]. FOOD SCIENCE, 2017, 38(9): 106-112.

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Degradation of Aflatoxin B1 by a Microbial Consortium and Its Composition and Diversity

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