Effect of Organic Solvent on Catalytic Kinetics of Polygalacturonase

doi:10.7506/spkx1002-6630-201205030

Abstract

Abstract: Water-soluble chitosan with low molecular weight was employed to modify polygalacturonase (PG). After modification, the catalytic kinetics of PG and modified polygalacturonase (COS-PG) were investigated in buffer, methanol, ethanol, acetone and tetrahydrofuran solution using pectin as substrate. The results indicated that Km of COS-PG revealed a slight decline, but Vmax revealed an increase. Km and Vmax of PG and COS-PG declined both in 2% methanol and 2% ethanol. Km and Vmax of PG and COS-PG increased in 2% tetrahydrofuran solution. Conversely, in 2% acetone solution, Km increased but Vmax declined.

Key words: polygalacturonase, organic solvents, dynamics

CLC Number:

Q643.132

LIU Xiao-lin，LI Xiao-ting，GONG Guo-yong，HUANG Lei，HUANG Zhuo-lie，WU Zhi-quan，LI Chun-yi. Effect of Organic Solvent on Catalytic Kinetics of Polygalacturonase[J]. FOOD SCIENCE, 2012, 33(5): 134-137.

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Effect of Organic Solvent on Catalytic Kinetics of Polygalacturonase

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