Abstract: A new method based on matrix solid phase dispersion, ionic liquid-based homogeneous liquid-liquid microextraction and high performance liquid chromatography was developed for the determination of fluroquinolones (FQs), including enoxacin (ENO), pefloxacin (PEF), norfloxacin (NOR) and enrofloxacin (ENR) in muscle samples. Muscle samples were directly treated by matrix solid phase dispersion using silica gel as dispersant, 200 μL of 1-hexyl-3- methylimidazolium hexafluorophosphate ([C6mim]BF4) was used as extraction solvent, and aqueous solution of pH 1.0 was used as elution solvent. After the target analytes were transferred into eluate, ammonium hexafluorophosphate was added. The eluate obtained was treated by ionic liquid-based homogeneous liquid-liquid microextraction and the analytes were enriched into the ionic liquid phase. High performance liquid chromatography with diode array detection was applied to separate and determine the target analytes. The experimental results showed that the calibration curve exhibited a good linear relationship (r > 0.997 4). The limits of detection (LODs) of the method were in the range of 2.9 to 8.6 μg/kg. The recoveries were between 87.9% and 105.3% with relative standard deviations (RSDs) of 2.2%–8.6%. The proposed method has the advantages of simple operation and no need for organic solvent and can be extensively applied for determination of fluroquinolones in animal tissue samples.

Key words: matrix solid phase dispersion, ionic liquid-based homogeneous liquid-liquid microextraction, fluroquinolones, muscle samples, high performance liquid chromatography (HPLC)