FOOD SCIENCE ›› 2017, Vol. 38 ›› Issue (18): 8-16.doi: 10.7506/spkx1002-6630-201718002

• Bioengineering • Previous Articles     Next Articles

Optimization of Bacterial Cellulose Production by Fermented Soybean Molasses with Komagataeibacter intermedius

YIN Yuan, MA Jiage, NI Chunlei, CHENG Jianjun, XU Su   

  1. (1. College of Food Science, Northeast Agricultural University, Harbin 150030, China;2. Harbin Light Industry College, Harbin 150000, China)
  • Online:2017-09-25 Published:2017-09-04

Abstract: In this study, Komagataeibacter intermedius CGMCC12562 was employed to produce bacterial cellulose (BC) from soybean molasses. The fermentation process was optimized to improve BC yield. Through one-factor-at-a-time experiments, the soluble solids content (SSC) of soybean molasses, and corn gluten meal, ZnSO4 and malic acid were selected as the factors with a significant influence on BC production for optimization by response surface methodology. It was found that the optimized culture medium consisted of soybean molasses with an SSC level of 14.13 °Brix, 1.6% corn gluten meal, 0.11% ZnSO4 and 0.41% malic acid. The BC yield was as high as (15.68 ± 0.82) g/L after 7 days of culture at 30 ℃ for in a 250-mL conical flask containing 16% of the medium with an inoculum size of 10% at an initial pH of 6.0 under static?cultivation?conditions.

Key words: soybean molasses, bacterial cellulose, Komagataeibacter intermedius, medium, optimization

CLC Number: