Optimization of Preparation of Angiotensin Converting Enzyme (ACE) Inhibitory Peptide RLSFNP Liposomes

doi:10.7506/spkx1002-6630-201720020

Abstract

Abstract: The objective of the present study was to optimize the preparation of liposomes incorporating the angiotensin converting enzyme (ACE) inhibitory peptide Arg-Leu-Ser-Phe-Asn-Pro (RLSFNP) using combination of one-factor-at-a-time method and response surface methodology. The response variable was the entrapement efficiency. The optimum preparation conditions were determined as follows: soy lecithin, 120.0 mg; cholesterol, 24.6 mg; RLSFNP, 15.3 mg; ether, 15 mL; PBS buffer (pH 7.4), 5.8 mL; and ultrasonication time, 5.2 min. Under these conditions, the average diameter, potential and entrapement efficiency of liposomes were 168 nm, –31.2 mV and (67.5 ± 0.8) %, respectively. The liposomes could sustainably release RLSFNP.

Key words: angiotensin converting enzyme (ACE) inhibitory peptides, liposomes, entrapement efficiency, bioavailability

CLC Number:

TS252.1

GUO Yuxing, XUE Yiqiu, JIANG Xiaoxiao, WU Zhen, ZENG Xiaoqun, SUN Yangying, PAN Daodong. Optimization of Preparation of Angiotensin Converting Enzyme (ACE) Inhibitory Peptide RLSFNP Liposomes[J]. FOOD SCIENCE, 2017, 38(20): 139-145.

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Optimization of Preparation of Angiotensin Converting Enzyme (ACE) Inhibitory Peptide RLSFNP Liposomes

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