Analysis of Virus Diversity in Drinking Source Water by Using Illumina MiSeq Sequencing Technology

doi:10.7506/spkx1002-6630-201802045

Abstract

Abstract: The viruses in drinking source water from east Tai Lake were separated and concentrated by gradient-series connection-circulation-tangential flow ultrafiltration (GSC-TFF). The viral genome was amplified by sequence independent single primer amplification (SISPA), sequenced by Illumina Miseq, and compared with the NCBI gene database using the basic local alignment search tool (BLAST). After quality control, 1 190 914 928 bp gene data were obtained, which could be assembled into 5 554 scaffold sequences. Viral genome function was found and annotated to Caudovirales, Herpesvirales and Ligamenvirales at the family level. A total of 40 families of homologous sequences were annotated, Microviridae (27.590 1%), Siphoviridae (23.010 7%), Phycodnaviridae (5.322 2%), Retroviridae (1.691 2%), Mimiviridae (1.960 8%) being the dominant ones. A total of 102 112 reads (21.572 8%) were identified as no rank at the family level. The approach proposed in this study can allow high throughput analysis of virus diversity in source water, paving the foundation for?detecting virus in water.

Key words: Illumina Miseq sequencing, drinking source water, virus, diversity

CLC Number:

Q939.9

GE Yingliang, YU Shuili, SHI Wenxin. Analysis of Virus Diversity in Drinking Source Water by Using Illumina MiSeq Sequencing Technology[J]. FOOD SCIENCE, 2018, 39(2): 287-292.

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Analysis of Virus Diversity in Drinking Source Water by Using Illumina MiSeq Sequencing Technology

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