Preparation of Conjugated Linoleic Acid Glyceride by Direct Esterification under Supercritical Carbon Dioxide

doi:10.7506/spkx1002-6630-201108007

Abstract

Abstract: The lipase-catalyzed preparation of conjugated linoleic acid glyceride (CLA) from conjugated linoleic acid and glycerin under supercritical CO2 was studied. The effects of the quantities of molecular sieve and enzyme addition, pressure, temperature and time on the esterification rate of CLA were investigated. The results showed that the optimal parameters for preparing CLA were as follows: in the attendance of 6% type 3A molecular sieve and 4% Novozym 435 lipase, reaction at 60 ℃ and 11 MPa for 20 h. Under these conditions, the esterification rate of CLA reached 90.98%. The contents of 9c,11t-CLA and 10t,12c-CLA was 37.79% and 41.66%, respectively in the CLA glyceride prepared.

Key words: supercritical CO2, conjugated linoleic acid (CLA), glyceride, esterification rate of CLA

CLC Number:

TS229

LI Mo-xin，LIU Jing，ZHOU Xiao-dan，GAO Yu-wei，YU Dian-yu. Preparation of Conjugated Linoleic Acid Glyceride by Direct Esterification under Supercritical Carbon Dioxide[J]. FOOD SCIENCE, 2011, 32(8): 29-32.

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Preparation of Conjugated Linoleic Acid Glyceride by Direct Esterification under Supercritical Carbon Dioxide

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