Isolation and Identification of Anthocyanins in the Fruits of Prunus humilis Bunge

doi:10.7506/spkx1002-6630-201109013

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (9): 59-63.doi: 10.7506/spkx1002-6630-201109013

• Basic Research • Previous Articles Next Articles

Isolation and Identification of Anthocyanins in the Fruits of Prunus humilis Bunge

CHANG Hong1，LAN Yan-ping1,*，ZHOU Jia-hua1，HE Hong-ju2，GAO Hua-jie3， FENG Cui-ping4

1. Institute of Agricultural Integrated Development, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China； 2. National Engineering Research Center for Vegetables, Beijing 100097, China； 3. College of Life Science, Capital Normal University, Beijing 100048, China； 4. College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, China

Online:2011-05-15 Published:2011-04-11

Abstract

Abstract: The objective of this study was to isolate and identify the anthocyanins in the fruits of Prunus humilis Bunge by high performance liquid chromatography-electrospray ionization tandem mass spectrometry. Six compounds were isolated and identified to be cyaniding-3-glucoside, geranium-3-glucoside, cyanidin-3-rhamnosyl glucoside, geranium-3-rhamnose glucoside, cyanidin-3-acetyl glucoside and geranium-3-acetyl glucoside. Among them, cyaniding-3-glucoside was the major anthocyanin, accounting for 62.6% of the total anthocyanins. In conclusion, high performance liquid chromatography-electrospray ionization tandem mass spectrometry can efficiently analyze the composition of anthocyanins in the fruits of Prunus humilis Bunge.

Key words: Prunus humilis Bunge, anthocyanins, isolation, identification, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS)

CLC Number:

O657.63

CHANG Hong，LAN Yan-ping，ZHOU Jia-hua，HE Hong-ju，GAO Hua-jie， FENG Cui-ping. Isolation and Identification of Anthocyanins in the Fruits of Prunus humilis Bunge[J]. FOOD SCIENCE, 2011, 32(9): 59-63.

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Isolation and Identification of Anthocyanins in the Fruits of Prunus humilis Bunge

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