FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (9): 164-166.doi: 10.7506/spkx1002-6630-201109037

• Bioengineering • Previous Articles     Next Articles

Isolation and Identification of Weissella Strains in Chicken

JI Shu-juan1,LI Li1,WANG Jin-ling2,*   

  1. 1. College of Food Science, Shenyang Agricultural University, Shenyang 110161, China; 2. Shenyang Entry-Exit Inspection and Quarantine Bureau, Shenyang 110016, China
  • Online:2011-05-15 Published:2011-04-11

Abstract: The suspicious Weissella strains isolated from chicken were identified by 16S rDNA gene sequencing. Under sterile conditions, 10 dead broiler samples were collected from a certain chicken farm in Shenyang. The bacteria in each sample were isolated and purified under micro-aerobic conditions. Totally, 14 bacterial strains were obtained and identified as Weissella. PCR based 16S rDNA gene sequencing showed that X1001, X1002, X1006, X1008 and X1009 strains had high homology (above 90%). The biochemical experimental results revealed that X1006 strain had the characteristics of Weissella confusa.

Key words: chicken, Weissella, isolation, identification, gene sequencing

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