Abstract: Objective: To investigate the effect of Pleurotus citrinopileatus polysaccharides (PCP) on antioxidant enzyme activities and isozyme profiles in the liver, heart and kidney of mice with carbon tetrachloride-induced acute liver injury. Methods:Mice were randomly divided into 6 groups: control group, model group, drug group (bifendate pills, 150 mg/(kg ·d)), low-dose PCP group (100 mg/(kg ·d)), medium-dose PCP group (200 mg/(kg ·d)) and high-dose PCP group (400 mg/(kg ·d)). All mice were administered for 30 consecutive days, and were fasted for 16 h after the end of the administration period before oral administration of CCl4 dispersed in salad oil for creating CCl4-inducedacute liver injury model. At 24 h postadministration, all mice were sacrificed to assay the activities of ALT, AST and antioxidant enzymes in the blood, liver, heart and kidney and analyze the isozyme profiles of CAT and SOD by gradientpolyacrylamide gel electrophoresis. Results: Compared with the control group, the model group exhibited a significant increase in the activities of serum ALT and AST and a significant decrease in the activities of the investigated antioxidant enzymes in the liver, heart and kidney of mice (P＜ 0.05 or 0.01 ). PCP administration resulted in a significant decrease in the activities of serum ALT and AST, and the medium-dose and high-dose PCP group exhibited significantly increased activities of CAT, GSH-Px and MnSOD in the liver, heart and kidney and could resume their normal levels. One CAT band was detected in the liver, heart and kidney and CCl4-induced acute liver injury resulted in a significant decrease in its staining intensity. In contrast, the staining intensity of the band was restored to the normal level after PCP administration. Similarly, One MnSOD and three CuZnSOD were detected in the liver and kidney, and PCP administration resulted in an increase in the staining intensity of total SOD and MnSOD, one MnSOD and two CuZnSOD were detected in the heart and the staining intensity of the bands were increased to their normal levels after PCP administration. Conclusions: PCP can significantly decrease the levels of serum ALT and AST, enhance the activities of antioxidant enzymes in the liver, heart and kidney of mice and promote the expression of their isozymes, indicating that PCP protects the major visceral organs of mice against carbon tetrachloride-induced acute liver injury.

Key words: Pleurotus citrinopileatus polysaccharides, acute liver injury, antioxidant enzymes, isozymes, gradient polyacrylamide gel electrophoresis