Preparation of an ELISA Method for Determining Buckwheat Allergen

doi:10.7506/spkx1002-6630-201120068

Abstract

Abstract: An indirect enzyme-linked immunosorbent assay (IELISA) was proposed to detect specific antibodies in tartary buckwheat allergenic patients, sera using polystyrene micro-well plate coated with natural tartary buckwheat allergenic proteins and horseradish peroxidase (HRP)-labeled mouse anti-human IgE. Key operations and reaction conditions were optimized. The results showed that the optimal coating antigen concentration, positive serum dilution, HRP-labeled mouse anti-human IgE concentration, blocking time at 37 ℃, reaction time between coating antigen and antibodies in positive sera, and reaction time between positive sera and HRP-labeled mouse anti-human IgE were 100 μg/mL, 1:50, 1:1000, 40 min, 40 min and 40 min, respectively. This method proved highly specific, repeatable and sensitive.

Key words: tartary buckwheat, allergen, kit, indirect enzyme-linked immunosorbent assay (IELISA)

CLC Number:

R392.11

ZHANGXin，CUIXiao-dong，WANGZhuan-hua. Preparation of an ELISA Method for Determining Buckwheat Allergen[J]. FOOD SCIENCE, 2011, 32(20): 327-330.

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Preparation of an ELISA Method for Determining Buckwheat Allergen

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