FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (24): 83-87.doi: 10.7506/spkx1002-6630-201124018

• Processing Technology • Previous Articles     Next Articles

Isolation of Glutathione from Aqueous Extract of Defatted Wheat Germ

SONG Guo-hui,HUANG Ji-nian,SUN Qiang,ZHANG Li-xia,LU Xin   

  1. (Institute of Agricultural Products Processing, Henan Academy of Agriculture Sciences, Henan Engineering Research Centre of Bioactive Substances in Agricultural Products, Zhengzhou 450002, China)
  • Online:2011-12-25 Published:2011-12-29

Abstract: The isolation of glutathione (GSH) from aqueous extract of defatted wheat germ (DWG) was explored. The effects of temperature and pH on the stability of GSH in aqueous extract of DWG were studied during. An isolation method for isolating GSH from aqueous extract of DWG was established using ultra-filtration followed by TOSO SP-650M ion-exchange resin chromatography. The GSH content in the isolated product was 8.8% compared with 0.79% in aqueous extract of DWG.

Key words: defatted wheat germ, glutathione, ultra-filtration, ion-exchange

CLC Number: