Abstract: In order to exploit the resource of Rana dybowskii eggs, protamex was used to hydrolyze egg protein into small peptides in this study. The degree of hydrolysis of Rana dybowskii eggs was investigated with respect to hydrolysis parameters such as pH, enzyme dose, solid-to-liquid ratio, hydrolysis time and temperature. The optimal hydrolysis parameters were determined as follows: pH 8.0, 3000 U/g pro of enzyme dose, 55 ℃, 1:5.5 (m/V) of solid-to-liquid ratio and 5 h of hydrolysis time. The hydrolysate obtained under these conditions was determined to have obvious anti-fatigue function in mice in terms of weight-loaded swimming time and the contents of blood lactic acid and liver glycogen.

Key words: Rana dybowskii, egg protein, hydrolysis, anti-fatigue activity