FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (10): 78-83.doi: 10.7506/spkx1002-6630-201210016

• Processing Technology • Previous Articles     Next Articles

Separation of Anthocyanins from Wild Bilberry by HPD-700 Macroporous Resin

LU . . Chun-mao1,2,BAO Jing2,MENG Xian-jun2,DONG Wen-xuan1,*,WANG Xin-xian2   

  1. (1. College of Horticultural Science, Shenyang Agricultural University, Shenyang 110866, China; 2. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China)
  • Online:2012-05-25 Published:2012-05-07

Abstract: In this study, the separation methods of anthocyanins from wild bilberry were investigated. The results showed that the macroporous resin HPD-7 was the optimal absorption material. The optimum conditions of adsorption and desorption were pH 2.0, anthocyanins concentration of 0.75 mg/mL, sample loading volume of 22 BV (resin bed volume) the adsorption speed of 0.5 mL/min, the 60% ethanol volume as the eluent of 5 BV, and the desorption speed of 1.5 mL/min. Under the optimal separation and purification conditions, a purplish dark powder with color value of 62.40 was achieved at the recovery rate of 86.20%.

Key words: wild bilberry, HPD-700 macroporous resin, anthocyanins, adsorption, desorption

CLC Number: