Physico-chemical Properties of Sika Deer Antler Collagen

doi:10.7506/spkx1002-6630-201211017

Abstract

Abstract: The physico-chemical properties of sika deer antler collagen extracted in acidic solution with pepsin were determined using ultraviolet (UV) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, amino acid analyzer, SDS-PAGE and differential scanning calorimetry (DSC). A typical absorption peak at 230 nm was found in the UV spectrum. The FTIR spectrum showed that the amide A, B and Ⅰ spectral bands were consistent with those standard typeⅠ collagen with three-spiral structure. SDS-PAGE results indicated that sika deer antler collagen was composed of one β-chain and two different α-chains (α1 and α2), which were the characteristics of type Ⅰ collagen. Amino acid composition analysis revealed the characteristic amino acids of typeⅠ collagen. The ts was 84.05 ℃. On the basis of the analysis above, it could be concluded that sika deer antler collagen had typical physico-chemical properties of type Ⅰ collagen.

Key words: sika deer antler, collagen, macromolecular protein, structural characteristics, physico-chemical properties

CLC Number:

Q816

ZHAOYu-hong，JINXiu-ming. Physico-chemical Properties of Sika Deer Antler Collagen[J]. FOOD SCIENCE, 2012, 33(11): 75-78.

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Physico-chemical Properties of Sika Deer Antler Collagen

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