Molecular Weight Changes and Kinetics of Acid Hydrolysis of Exopolysaccharides Isolated from Cordyceps sinensis

doi:10.7506/spkx1002-6630-201211019

Abstract

Abstract: In this study, exoplysaccharides were isolated from Cordyceps sinensis. Gel permeation chromatography (GPC) was used to determine molecular weight (MW) and molecular weight distribution of exopolysaccharides subjected to acid hydrolysis. The results showed that high molecular weight fraction (HMWF) and low molecular weight fraction (LMWF) of exopolysaccharides were obtained by acid hydrolysis. The content, MW and polydispersity index (PDI) of HMWF decreased with increasing reaction time, but the molecular weight degradation ratio (MWDR) showed a linear increase. On the other hand, the content of LMWF increased with increasing reaction time, with mostly a molecular weight of 3.0 kD. The MWDR of LMWF showed a linear increase within the first half an hour of reaction. Afterwards, the MWDR kept constant at about 0.25. Acid hydrolysis of exopolysaccharides was systemetic scission, which followed the pseudo first-order kinetics. The obtained value of rate constant k was 3.24 × 10-2min-1, which was much less than that of random scission.

Key words: Cordyceps sinensis, exopolysaccharides, acid hydrolysis, molecular weight, kinetics

CLC Number:

TS201.2

YANJing-kun，WUJian-yong，JINBei，CUIHai-ying. Molecular Weight Changes and Kinetics of Acid Hydrolysis of Exopolysaccharides Isolated from Cordyceps sinensis[J]. FOOD SCIENCE, 2012, 33(11): 82-85.

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Molecular Weight Changes and Kinetics of Acid Hydrolysis of Exopolysaccharides Isolated from Cordyceps sinensis

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