Multiplex PCR Detection of Transgenic Components of Genetically Modified Rice

doi:10.7506/spkx1002-6630-201212031

FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (12): 159-162.doi: 10.7506/spkx1002-6630-201212031

• Analysis & Detection • Previous Articles Next Articles

Multiplex PCR Detection of Transgenic Components of Genetically Modified Rice

WEI Shuang1，CHEN Zhen1，LU Chun-bin2，MA Jun3，BAI Wei-bin1，WU Xi-yang1,*

(1.Department of Food Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou 510632, China； 2. Institute of Reproductive Immunology, Jinan University, Guangzhou 510632, China；3. Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China)

Online:2012-06-25 Published:2012-07-27

Abstract

Abstract: In order to detect transgenic components in genetically modified rice, endogenous gene sps, exogenous insect resistant gene Cry1Ab/Ac, exogenous insect resistant gene Cry1Ab, exogenous insect resistant gene Btc, reporter gene gus, nos terminator and CaMV35S promoter were evaluated by multiplex polymerase chain reaction. Seven pairs of primers were designed, and primer concentration and annealing temperature were optimized to establish a multiplex PCR system. The results showed that the developed seven-plex PCR system could allow the detection of transgenic components in genetically modified rice and other crops including soybean, corn, cottonseeds and rapeseed meal with high efficiency and good stability.

Key words: transgenic detection, rice, seven-plex PCR

CLC Number:

TS207.7

WEI Shuang，CHEN Zhen，LU Chun-bin，MA Jun，BAI Wei-bin，WU Xi-yang. Multiplex PCR Detection of Transgenic Components of Genetically Modified Rice[J]. FOOD SCIENCE, 2012, 33(12): 159-162.

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Multiplex PCR Detection of Transgenic Components of Genetically Modified Rice

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