FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (14): 249-258.doi: 10.7506/spkx1002-6630-20180821-218

• Composition Analysis • Previous Articles     Next Articles

Saponins in Panacis japonici Rhizoma as Analyzed by UFLC-Triple TOF MS/MS

CHEN Jiali, TAN Mengxia, ZOU Lisi, LIU Xunhong, CHEN Shuyu, SHI Jingjing, WANG Chengcheng, MEI Yuqi   

  1. School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
  • Online:2019-07-25 Published:2019-07-23

Abstract: In this study, we analyzed the saponins of Panacis japonici Rhizoma by ultra-fast liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry (UFLC-Triple TOF MS/MS). The chromatographic separation was performed on a SynergiTM Hydro-RP 100 ? (2.0 mm × 100 mm, 2.5 μm) with gradient elution using a mobile phase comprised of 0.1% formic acid solution (A)-0.1% formic acid acetonitrile (B). MS data were collected in the negative ion mode. Based on the accurate molecular mass information of quasi-molecular and fragment ions provided by highresolution mass spectrometry combined with standard reference data and relevant literature data, a total of 53 saponins were finally identified, including 18 glycosides of protopanaxadiol (PPD), 21 glycosides of protopanaxatriol (PPT), 10 glycosides of oleanolic acid and 4 glycosides of ocotillone. This study paves the way for exploring the pharmacodynamic material basis of P. japonici Rhizoma and developing a comprehensive quality evaluation system.

Key words: Panacis japonici Rhizoma, UFLC-Triple TOF MS/MS, saponins

CLC Number: