Abstract: To investigate the influence of peptide derived from naked oat globulin (PNOG) on antioxidant capacity in aging mice, a subacute aging mouse model was established by neck back subcutaneous injection of D-galactose (120 mg/(kg mb·d)) once daily for 6 consecutive weeks. Healthy male mice were randomly divided into 6 groups: normal control group, aging model group, low-, medium- and high-dose PNOG groups (200, 600, 1 000 mg/(kg mb·d)) and vitamin C (VC, positive control) group (100 mg/(kg mb·d)). After 6 weeks, body mass, liver index and brain index were examined and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) were measured in the serum of mice. The activities of GSH-Px and monoamine oxidase B (MAO-B) and malondialdehyde (MDA) content were measured in liver and brain tissues. The results showed that body mass in the aging model group increased more slowly, and liver and brain indexes decreased; the activities of SOD, GSH-Px and CAT in serum significantly declined (P < 0.05), and so did GSH-Px activity in liver and brain tissues (P < 0.05), but MAO-B activity and MDA content significantly increased as compared to the normal control group (P < 0.01). For the aging mice treated with PNOG at medium and high doses, body mass increased fast, and liver and brain indexes became greater. The activities of SOD and GSH-Px in serum as well as GSH-Px activity in liver and brain tissues significantly increased, and MAO-B activity and MDA content significantly declined (P < 0.01) as compared to aging model mice. These data indicated that PNOG could effectively remove free radicals, and significantly increase antioxidant capacity in D-gal-induced aging mice in a dose-dependent manner.

Key words: naked oats, globulin, peptide, antioxidant, aging