Screening of a High-Yield of γ-Ployglutamic Acid-Producing Strain by Means of Both UV Light and He-Ne Laser

doi:10.7506/spkx1002-6630-201213039

FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (13): 183-186.doi: 10.7506/spkx1002-6630-201213039

• Bioengineering • Previous Articles Next Articles

Screening of a High-Yield of γ-Ployglutamic Acid-Producing Strain by Means of Both UV Light and He-Ne Laser

QIAOChang-sheng，LIXue，LANLing-fang，CHENXiao，MAZheng-wang，LIZheng

(1. Tianjin Key Laboratory of Industry Microbiology, Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China；2. Tianjin Peiyang Biotrans Biotech Co. Ltd., Tianjin 300457, China；3. School of Textiles, Tianjin Polytechnic University, Tianjin 300160, China)

Online:2012-07-15 Published:2012-07-27

Abstract

Abstract: Bacillus licheniformis BCPG006 was mutagenized by means of both UV and He-Ne laser. A mutant strain named as Bacillus licheniformis BCPG078 having a high ability to produce γ-polyglutamic acid (γ-PGA), was obtained by resistance screening. The γ-PGA yield of the mutant strain was 16 g/L during 72 h of shake flask culture at 37 ℃, initial pH 7 and 200 r/min, which was 2.67-fold higher than that obtained from the original strain (6 g/L). These results suggest that the mutant strain has strong genetic stability. Meanwhile, combination of UV and He-Ne laser provides an effective method for the screening of γ-PGA-producing strains.

Key words: γ -PGA, compound mutation, resistant plate, fermentation condition

CLC Number:

Q815

QIAOChang-sheng，LIXue，LANLing-fang，CHENXiao，MAZheng-wang，LIZheng. Screening of a High-Yield of γ-Ployglutamic Acid-Producing Strain by Means of Both UV Light and He-Ne Laser[J]. FOOD SCIENCE, 2012, 33(13): 183-186.

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Screening of a High-Yield of γ-Ployglutamic Acid-Producing Strain by Means of Both UV Light and He-Ne Laser

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