Abstract: In this experiment, 15 strains of Lactobacillus were studied for their inhibition on HT-29 cell proliferation, and 8 of these were found to have strong inhibition effect and they were texted for their adhesion to HT-29 cells and antioxidant capacity. The results showed that the inhibition rates of the selected strains against the proliferation of HT-29 cells ranged from 10.02% to 15.63%, and the adhesion rates to HT-29 cells ranged from 3.9% to 19.1%. The reducing capacity of the live bacterial suspensions, cell lysates, and fermentation supernatants were 23.43–271.76 μmol/L L-cysteine hydrochloride, and the scavenging rates against 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl and superoxide anion radicals were 10.89%–50.42%, 7.46%–33.16%, and 17.64%–46.13%, respectively, and the inhibition rate against lipid oxidation were 10.45%–51.91%. Principal component analysis showed that L. acidophilus KLDS1.0901 had the best comprehensive performance, followed by L. acidophilus KLDS1.1003. The two strains had good tolerance to simulated gastrointestinal fluids. After digestion, the viable count could still be above 108 CFU/mL, and the survival rate was 50%. In general, L. acidophilus KLDS1.0901 and KLDS1.1003 had a strong ability to inhibit HT-29 cell proliferation and possessed good probiotic properties with L. acidophilus KLDS1.0901 being more effective.

Key words: Lactobacillus, inhibition, adhesion, antioxidant, tolerance