FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (14): 138-145.doi: 10.7506/spkx1002-6630-20190719-247

• Bioengineering • Previous Articles     Next Articles

Optimization of Cleaning-in-place Regime for Removal of Bacillus cereus Biofilm

HUANG Ziyu, LIN Yingying, SONG Sijia, REN Fazheng, GUO Huiyuan,   

  1. (1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing 100083, China; 2. Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Government, China Agricultural University, Beijing 100083, China)
  • Published:2020-07-29

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Abstract: In this study, using an orthogonal array design, the optimum cleaning-in-place (CIP) regime for thoroughly removing the biofilm of Bacillus cereus A1, which has excellent biofilm-forming ability and high alkali and acid tolerance, on stainless steel sheets was determined as sequential washing with 17.5 g/L NaOH solution at 85 ℃ for 20 min, water, 15 g/L HNO3 at 85 ℃ for 20 min, and water once again. To reduce their use, acid and alkali cleaning agents were used in combination with peracetic acid, and the optimized CIP protocol was determined as sequential washing with 15.0 g/L NaOH solution at 85 ℃ for 10 min, water, 10.0 g/L HNO3 at 85 ℃ for 10 min, 3 g/L peracetic acid at 25 ℃ for 10 min, and neutralizer. This CIP protocol could not only thoroughly remove Bacillus cereu, but also reduce the concentration of acid and alkali cleaning agents and the cleaning time, providing a more efficient and eco-friendly method to clean food processing equipment.

Key words: cleaning-in-place; Bacillus cereus; biofilm; orthogonal array design; peroxyacetic acid

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