Fully 13C Isotope Labeled Internal Standard for the Accurate Quantification of Zearalenone in Cereals and Oils by Ultra-high Performance Liquid Chromatography-Tandem Triple Quadrupole Mass Spectrometry after Automated Purification with Immunoaffinity Column

doi:10.7506/spkx1002-6630-20190927-339

FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (24): 267-272.doi: 10.7506/spkx1002-6630-20190927-339

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Fully 13C Isotope Labeled Internal Standard for the Accurate Quantification of Zearalenone in Cereals and Oils by Ultra-high Performance Liquid Chromatography-Tandem Triple Quadrupole Mass Spectrometry after Automated Purification with Immunoaffinity Column

WU Yu, YE Jin, LI Li, LI Sen, XUAN Zhihong, CUI Hua, LIU Hongmei, WANG Songxue, MA Haihua, ZHANG Feng, HE Jianhong

(1. Institute of Grain and Oil Quality Safety, Academy of National Food and Strategic Reserves Administration, Beijing 102629, China; 2. College of Information Science and Engineering, Henan University of Technology, Zhengzhou 450001, China; 3. Raykol Instrument Co. Ltd., Xiamen 361000, China)

Online:2020-12-25 Published:2020-12-28

Abstract

Abstract: An accurate method for the quantification of zearalenone in cereals and oils was developed by automated purification with immunoaffinity column combined with ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry (UPLC-MS/MS) using a fully 13C isotope labeled standard. The results showed that the quantification limit (LOQ) and detection limit (LOD) of the internal standard method were 0.33 and 1 μg/kg, respectively, with a correlation coefficient R2 of 0.999 5. The recoveries were 92.7%–101.3% with relative standard deviation (RSD) of 0.02%–3.74%. The results obtained by this method for three quality control samples were within the range of certified values. Its accuracy and precision were better than those of the external standard method. The internal standard method allowed the automated and efficient detection of massive samples without the need for nitrogen gas blowing and could avoid the errors caused by manual operations.

Key words: automated purification with immunoaffinity column; ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry; internal standard method; cereals and oils; zearalenone

CLC Number:

TS207.7

WU Yu, YE Jin, LI Li, LI Sen, XUAN Zhihong, CUI Hua, LIU Hongmei, WANG Songxue, MA Haihua, ZHANG Feng, HE Jianhong. Fully 13C Isotope Labeled Internal Standard for the Accurate Quantification of Zearalenone in Cereals and Oils by Ultra-high Performance Liquid Chromatography-Tandem Triple Quadrupole Mass Spectrometry after Automated Purification with Immunoaffinity Column[J]. FOOD SCIENCE, 2020, 41(24): 267-272.

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Fully 13C Isotope Labeled Internal Standard for the Accurate Quantification of Zearalenone in Cereals and Oils by Ultra-high Performance Liquid Chromatography-Tandem Triple Quadrupole Mass Spectrometry after Automated Purification with Immunoaffinity Column

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